A 3D hydrodynamic flow-focusing device for cell sorting

Abstract Optical-based microfluidic cell sorting has become increasingly attractive for applications in life and environmental sciences due to its ability of sophisticated handling flow. The majority these devices employ two-dimensional fluid flow control strategies, which lack the manipulate position cells arbitrarily precise optical detection, therefore resulting reduced accuracy purity. Although three-dimensional (3D) hydrodynamic have better flow-focusing characteristics, most flexibility sample each direction. Thus, there been very few studies using 3D focusing sorting. Herein, we designed a platform based on independent sheath pressure-actuated switching. This design offers many advantages terms reliable acquisition weak Raman signals precisely speed samples 3D. With proof-of-concept demonstration, show this focusing-based device potential reach high degree activated